Ultrafiltration operational conditions influence in the antibacterial activity of native and thermally treated lysozyme

To obtain high quality final products, biomolecules can be exposed in production lines to stress factors such as heating, stirring and multiple surfaces contact before their commercialization. These induce stability efficacy loss. In this work, the antibacterial activity of lysozyme (LSZ), a protein used antibiotic which acts by disrupting bacterial cell wall, was studied after two operations: heating ultrafiltration. Antibacterial extreme is expected decrease due conformational changes molecule. It observed that ultrafiltration promote an proportional applied transmembrane pressure non-treated lysozyme. However, when thermally treated (90 °C) filtered, no additional effect pore passage, independently pressure.